Queensland Facility for Advanced Genome Editing
The Queensland Facility for Advanced Genome Editing (QFAGE) provides expert genetic modification (GM) services using CRISPR/Cas9 genome editing and standard transgenic (TG) mouse production technologies.
Established in January 2016 at the Institute for Molecular Bioscience, QFAGE offers a flexible service to help life sciences and biomedical research groups make the most of this valuable technology.
QFAGE services are overseen by Professor Peter Koopman, whose lab has successfully produced dozens of TG and CRISPR/Cas9 models. The facility is managed by Dr Johnny Huang, who has several years of experience managing a commercial mouse TG and knockout mouse facility. Our combined experience assures a quality service that maximises experimental success.
Our standard CRISPR/Cas9 and TG mouse production service involves three staged modules.
- Pre-experiment design and reagents
- Microinjection, transfer and agistment to weaning stage
- Post-weaning analysis to handover stage
The cost for the standard service varies according to the type of experiment involved. In general, we provide the following types of services. Please contact us via the form below for detailed pricing.
Type 1 CRISPR/Cas9 project (simple): Gene knockout or large deletion. This normally involves targeting two sgRNAs either side of the region or exon to be deleted, and no repair template, so the deletion results from non-homologous end joining (NHEJ).
Type 2 CRISPR/Cas9 project (intermediate): Generation of small, specific changes (e.g. point mutations, small specific insertions/deletions up to 60bp, or epitope tagging). This normally involves using two sgRNAs targeted at the site of interest, and homology-director repair (HDR) via a single-stranded DNA oligonucleotide substrate (~150 bases).
Type 3 CRISPR/Cas9 project (complex): Generation of conditional (“floxed”) alleles or insertion of large DNA fragments. These experiments are much more complicated and involve either several sgRNAs (two for each LoxP insertion site) and repair templates, or a large plasmid DNA substrate (5-10kb) to either “flox” an exon or “knock-in” a reporter or other large piece of sequence.
TG mouse project: A standard project involves a simplified service module 1 (preparation of injectables), plus full service modules 2 and 3.
Customised TG services: A customised package consisting of service module 2 only (microinjection and embryo transfer) is available to approved users. Alternatively, a package of service modules 2 + 3 (microinjection, embryo transfer, supply of genotyped progeny) is also available. Approved users are those with demonstrated experience in supplying injection-quality TG construct DNA.
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Dr Johnny Huang
P 07 3346 2061
Prof Peter Koopman
P 07 3346 2059